Agilent Manuals

This Application Note describes the rapid separation of metabolites generated from in-vitro experiments using the Agilent 1290 Infinity LC system. It also explains the fast acquisition of TOF mass spectra using Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight LC/MS systems. The software-assisted identification of main metabolites from in-vitro experiments using Agilent MassHunter Metabolite Identification software is discussed. The generation of reports for the identified metabolites using Agilent MassHunter software is also covered.

This Application Note describes a high-speed, high-resolution capillary electrophoresis method of heparin and related impurities using highly concentrated buffer in a 25 µm bubble cell capillary. The method can be used to detect heparin and related impurities in a short time.

This document describes a one-color gene expression capability developed by Agilent Technologies, as well as the performance and advantages of this system.

Application Note Abstract Microarray-based gene expression hybridization is a powerful and proven technique for studying differential gene expression signatures in cancer. RNA extracted from freshly frozen tissues is optimal for microarray analysis; however, in many cases, formalin-fixed paraffi n-embedded (FFPE) tissues are the only samples available. To work with these diffi cult samples, Agilent has developed an FFPE sample protocol optimized for use with Agilent gene expression microarrays. To test the effectiveness of this protocol, total RNA was extracted from a quadruplicate set of colon tumor (adenocarcinoma), and matched normal colon FFPE and fresh frozen tissues. RNA purity and quality were determined, and the RNA was amplifi ed using a system in which a reverse transcription-generated cDNA library is subjected to limited PCR amplifi cation. The resulting cDNA target was chemically labeled. Analysis of the microarray results revealed a reduced sensitivity of detection for the FFPE-derived RNA samples; however, the lists of detected genes showed high overlap between the FFPE and frozen samples. Comparison of gene expression signals demonstrated excellent assay reproducibility between the technical repli- cates. Principal component analysis of array signals demonstrated clear separa- tion between the tumor and normal samples with grouping of replicate samples. Statistical analysis of the expression level variations between tumor and normal samples revealed that both the FFPE and frozen samples showed similar expression level changes.

This article introduces the application of the Agilent Technologies 6140 Single Quadrupole LC/MS High Throughput Mass Spectrometer. The Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system is used to analyze the impurities of octyl dimethyl para-aminobenzoic acid (OD-PABA). The instrument has a high scan speed capability, which allows clear detection of the degradation product p-dimethylbenzoic acid. The background and usage of OD-PABA are also discussed in this article.

Analysis of Aminoglycoside Antibiotics

This document describes the application of the Agilent Cell Viability Workstation and Promega CellTiter-Glo kit. The Agilent Cell Viability Workstation consists of a Molecular Devices SpectraMax M5 and an Agilent BenchCel Microplate Handling Workstation integrated with an Agilent Bravo Automated Liquid Handling Platform. This workstation is designed for automated cell viability testing and features a compact footprint and high-throughput processing capability. The Promega CellTiter-Glo kit utilizes a linear relationship between ATP concentration and light signal, measuring the ATP content in cells through the generation of luminescence to determine the number of viable cells.

The Agilent PCR Preparation Workstation is a flexible workstation that provides high-throughput PCR preparation. Up to 150 microplates can be processed in one run (with tip changing). The plate processing time is approximately 5 hours for a 50 microplate run, depending on the protocol complexity.

This document introduces the Agilent gDNA Isolation Workstation, which can efficiently extract DNA and can process up to 10 microplates at a time. The processing time for each microplate is approximately 10 minutes.

This application note describes the characterization of a monoclonal antibody at low nanogram levels using an Agilent HPLC-Chip system coupled to an Agilent Accurate-Mass Q-TOF MS instrument. The superior sensitivity and mass accuracy of the HPLC-Chip LC/MS platform, combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, enabled easy and rapid identifi cation of antibody heterogeneity and cleaved fragments. Robust, reliable, and easy to use, Agilent nanofl ow LC/MS technology is ideally suited for the routine analysis of biopharmaceuticals.

This document describes the applications of Agilent BenchCel Workstations Microplate Sealing Solution, including compound storage, screening, PCR/qPCR/rtPCR, and sequencing. An Automated Microplate Sealing Workstation can be created by integrating the BenchCel Microplate Handler and the Agilent PlateLoc Thermal Microplate Sealer, providing high throughput and easy operation.

This document describes the features and benefits of the Agilent BenchCel Workstations Automated Barcode Labeling Workstation Application Bulletin. The workstation offers high-speed plate transfers and labeling, scalability to integrate additional devices, and storage options for up to 320 microplates.

This document introduces the Agilent BenchCel Workstations Automated Microplate Centrifugation Solution, which can be used for PCR purification, cell harvesting, post-PCR process condensate spin-down, high density plate air bubble removal and many more applications. This solution integrates a wide range of instruments with the Agilent BenchCel Microplate Handler to create flexible benchtop automation solutions.