Agilent Manuals

This document describes the integration of Agilent ChemStation B.04.01 software with Agilent OpenLAB ECM software to meet the 21 CFR part 11 requirements

This paper compares the Agilent J&W DB-5ms Ultra Inert column with similar versions from four other column manufacturers with respect to their chromatographic behaviors for active analytes. Superior results were observed on the DB-5ms columns for basic compounds. Chromatograms depicting peak shape characteristics are presented to show how exceptional inertness leads to a significant reduction in tailing of active basic compounds for more accurate, reliable analytical results. Excellent chromato- graphic performance was obtained for a diverse group of compounds that included chlorophenols, organophosphorus pesticides and aromatic positional isomers demon- strating the applicability of the DB-5ms column for a wide range of applications.

This application note describes a method for quantifying three alkaloids in the stem of various mahonia species: berberine, palmatine, and jatrorrhizine. The method uses capillary electrophoresis (CE), which is particularly suited to the analysis of plant extracts because of its ability to deal with complex or “dirty” sample matrices.

The study proposes an optimized method for the analysis of tropane alkaloids by CE-ESI-MS. The results show that the method has high selectivity and sensitivity and is suitable for the quantitative analysis of tropane alkaloids in biological samples.

This paper describes the analysis of drugs of abuse using capillary electrophoresis coupled with electrospray ionization mass spectrometry (CE-ESI-MS). The unique identification of drugs was achieved by observing protonated molecular ions and characteristic fragment ions. The experimental results demonstrate the feasibility of the method, but additional method development and/or validation may be required for routine use.

This document describes the use of capillary electrophoresis coupled with electrospray ionization mass spectrometry (CE-ESI-MS) to analyze hallucinogenic mushroom extracts. The data presented here demonstrate feasibility. Additional method development and/or validation may be required for routine use.

This application note describes a method for the analysis of aromatic amines in leather extracts using CE-MS. This method provides specific identification of analytes in complex matrices that would present difficulties for HPLC. Organic extracts of leather fit this description exactly, therefore CE-MS is the method of choice.

This document describes the method and results of analyzing anions in power plant waters using capillary electrophoresis. Anion analysis is performed using indirect UV detection, allowing for fast and reliable analysis of low ppb levels of small inorganic and organic anions.

This Application Note describes methods of using the Agilent Capillary Electrophoresis (CE) system to separate single- and double-stranded DNA, single-stranded RNA and antisense oligonucleotides. Oligonucleotides with a chain length of 5 to 80 nucleotides are separated using polymer solution A and polymer solution B. The application note describes how to achieve high reproducibility in separations of oligonucleotides.

This document introduces a feasibility study of a capillary electrophoresis-mass spectrometry (CE-MS) technique for toxicological drug screening in blood samples. The study shows that the Agilent LC/MSD Trap system can detect drugs at levels as low as 20 ng/mL in whole blood, suggesting that it may be a suitable tool for screening whole blood samples for drugs. Additionally, the system's capability of generating MSn data allows reliable identification of detected drugs, suggesting that both screening and identification of drugs can be accomplished in one or two injections using the same instrument system.

This Application Note describes the use of highly sulphonated cyclodextrins for the analysis of chiral analytes. By using small 25 µm id capillaries with an extended pathlength of 125 µm id the Joule heating can be controlled while enhancing sensitivity.

This Application Note evaluates the performance of the Agilent 1290 Infinity LC system for the determination of impurities and related substances of metoclopramide hydrochloride in a pharmaceutical formulation. The translation of a conventional liquid chromatography (LC) method on a 1200 Series HPLC system to an ultra high pressure method on an Agilent 1290 Infinity LC system is discussed. Method translation is relatively easy and temperature fine-tuning is the most important parameter to obtain the same selectivity for the different impurities. Pressures as high as 1070 bar were applied during method development. The final high productivity method is carried out at 880 bar in an analysis time of 3.5 min which is approximately 4 times faster than the original HPLC method but with the same accuracy. A validation study was carried out to demonstrate the performance of the Agilent 1290 Infinity LC system. Limits of detection for the impurities were as low as 0.001 % w/w relative to the main compound using the new diode array detector (DAD). This is more than one order of magnitude lower than required.

This Application Note demonstrates: (1) The use of the Agilent 1200 Rapid Resolution LC (RRLC) system for high resolution separation of metabolites from an in-vitro metabolism experiment. (2) The use of the Agilent 6520 QTOF mass spectrometer for the acquisition of data for computer assisted metabolite identification. (3) The use of the Agilent MassHunter Metabolite identification software for highly productive identification of expected metabolites. (4) The results of the Metabolite ID data analysis for expected metabolites of the pharmaceutical drug nefazodone.

This Application Note demonstrates: The use of the Agilent 1200 Rapid Resolution LC (RRLC) system for high resolution separation of metabolites from an in-vitro metabolism experiment. The use of the Agilent 6520 QTOF mass spectrometer for the acquisition of data for computer assisted metabolite identification. The use of the Agilent MassHunter Metabolite identification software for highly productive identification of expected metabolites. The results of the Metabolite ID data analysis for expected metabolites of the pharmaceutical drug nefazodone