Agilent Manuals

This Application Note describes the experimental details and results of a plasma protein binding (PPB) assay. The approach and methodology of rapid resolution liquid chromatography and triple quadrupole mass spectrometry used in this study are described in a separate Application Note1. Experimental details and results of other typical assays per- formed in early ADME profiling of new hits are presented in separate publications2,3.

The method used SDB-RPS disk and FMOC for the detection of anatoxin-a. The recovery rate was 97.6%-102.1% at 10 ng/L, and the detection limit was 0.8 ng/L.

This file describes an LC/MSD autotuning method that can optimize analysis parameters in both positive and negative ionization modes and create a tuning file that contains optimized parameters.

A relatively rapid and sensitive analytical method for the quantitation of LSD, iso-LSD, and 2-oxo-3-hydroxy-LSD in urine using a single-quadrupole LC/MS system is reported.

The effects of four different ion-pair reagents (weak and strong volatile ion-pair reagents and nonvolatile strong ion-pair reagents) in positive and negative ionization modes are compared. The results show that the strength and volatility of the ion-pair reagent have a great influence on the formation of the ion pair and the resulting ion-pair effect. Strong volatile ion-pair reagents show good performance in both positive and negative ionization modes, while nonvolatile strong ion-pair reagents show good performance in positive ionization mode.

This document introduces the characteristics of the Agilent 1100 Series LC/MSD electrospray interface, including system composition, working principle, application range, etc.

Introduce a method for coupling nano-LC with MALDI mass spectrometry, which allows to deposit the LC run on the MALDI plate in real time, and thus couple the high resolution power of nano-RP-HPLC separation directly with MALDI mass spectrometry and decouple the aquisition of MS/MS from chromatographic separation and MS cycle time.

This article compares the detection techniques UV, fluorescence, and mass spectrometry in combination with standard and capillary LC systems and columns with different internal diameters. The advantages and disadvantages of the different detector/column/LC system combinations are discussed.

This application note presents the procedure for the analysis of morphine and its metabolites in blood plasma using electrospray LC/MS, a method which does not require derivatization.

The document discusses the challenge of analyzing minor impurities in partially decomposed samples using liquid chromatography/mass spectrometry (LC/MS) technique. It demonstrates the use of Agilent 1100 Series LC/MSD SL quadrupole mass spectrometer with ChemStation software to improve the detection of impurities. The experiment was conducted using a partially decomposed sample of the bronchodilator salbutamol. The application of data analysis and software tools enhanced the ability to detect impurities.

This is an application note describing a method for quantification of digoxin using liquid chromatography-mass spectrometry (LC-MS) in human serum

A fast, sensitive and reproducible technique for confirming the presence of drugs of abuse (DOA) in oral fluids (OF) using the Agilent G6410AA Triple Quadrupole Mass Spectrometer (QQQ) is presented.

This Application Note will demonstrate the use of the Agilent 1200 Series Rapid Resolution LC system with Rapid Resolution High Throughput (RRHT) columns for the separation of the ingredients found in a complex ginseng root extract. The information obtained with an ion trap MSn and MRM is used to determine the structure of the ingredients and for quality control purposes.

This Application Note describes an antibody-based method for identifying and counting blood cells from whole blood samples on the Agilent 2100 bioanalyzer without a washing step. The ability of the Agilent 2100 bioanalyzer to identify peripheral whole blood cells is demonstrated and compared to a flow-cytometry-based reference instrument.

This document introduces the ZORBAX Extend-C18 columns from Agilent Technologies, which can be used for separating explosive compounds. The columns offer various configurations for customized HPLC methods based on resolution, speed, and solvent usage. The document also highlights the advantages of different column configurations, including resolution, speed, and solvent savings. By simply substituting the column, users can choose the desired combination of resolution, speed, and solvent usage.