Agilent Manuals

This application note describes the steps involved in measuring the cover and shade protection factors of synthetic shadecloth. The method is based on measuring the absorption of UVR by the shadecloth samples using a spectrophotometric method, from which the shade protection factor is determined.

This document describes a quantitative analysis method for complex multi-component mixtures, focusing on the features and advantages of Agilent Technologies, Inc.'s Cary multi-component software.

This application note describes an automated analysis method for determining the uniformity of dosage in quinine sulfate tablets using an Agilent fiber-optic autosampler. The method uses a Cary 3C double beam UV/Vis spectrophotometer, Agilent SPS-5 autosampler, Cary fiber probe, and Cary fiber optic coupler. A Cary OS2 concentration application module and simple ADL program are used to locate the peak maximum and its absorbance value, from which the concentration of the analyte can be determined from known standards or a known extinction coefficient. This method has been applied to the measurement of uniformity of dosage for quinine sulfate in tablets using the USP procedure.

This document introduces how to use fluorescein to detect the effects of trial drugs on the activity of trypanothione reductase.

This application note describes how to use a LINKAM THMS600 hot-stage as a sample holder connected to a Cary 5 UV-Vis-NIR spectrophotometer to obtain temperature-dependent UV-Vis absorption spectra of polymer thin films.

This document introduces a new automated plant pigment analysis system. The system uses spectrophotometric techniques to analyze plant pigments. It can accurately detect the concentration and composition of plant pigments.

This article describes the use of an optical fiber attachment for spectro-electrochemical studies. The reduction of CoPc in DMF and its reaction with CO2 were investigated using an optical fiber probe. The advantages of using an optical fiber probe include flexibility, convenience, and suitability for electro-catalysis research.

This document introduces the Imaging technology, including the definition and implementation of Imaging.

This application note introduces the evolution of infrared (IR) microscopy, with a focus on Agilent's Agilent IR Microscope (IRM) 4000 and Agilent IR Microscope (IRM) 5000.

The characterization of optical components using the Cary Deep UV spectrophotometer and 'VW' specular reflectance accessory

This document introduces the application of ATR-FTIR spectroscopy for quantitative analysis. The use of ATR accessories with Fourier transform infrared (FTIR) spectrometers is now commonplace. This accessory allows for the non-destructive measurement of samples with little or no preparation. Most samples can be directly applied to the internal reflection element (IRE) of an ATR without time-consuming dilution with matrices such as Nujol or KBr. The ATR accessory also allows for easy analysis of liquid samples with just a single drop required, applied directly to the IRE crystal. However, by the nature of their design, ATR accessories absorb infrared radiation and consequently reduce the amount of energy that reaches the infrared detector. The attenuation caused by these accessories typically varies from 70–90%. Agilent Cary 600 FTIR series spectrometers provide the ideal platform for this accessory by delivering up to four times more IR power at the sample focus than most competitive products. They have been designed to offset accessory attenuation of the IR beam and to provide exceptional performance and sensitivity, which are typically compromised with the use of the accessory. Infrared spectroscopy is particularly well-suited to quantitative analyses. The determination of sample concentration is made simple with the Quant Calibration application in the Resolutions Pro software.

The application note introduces the technique of using the Agilent Cary 670 FTIR spectrometer to observe rotational and isotopic bands in CO.

Multi-frequency homonuclear decoupling is a decoupling technique that uses multiple frequencies to decouple multiple multiplets simultaneously, which can significantly speed up the decoupling process and improve experimental efficiency.

This application note describes the experimental steps for measuring the hybridization between a quencher (DABCYL) labeled PNA probes and a fluorescein labeled DNA using the Fluorescence BioMelt Package