Agilent Manuals

Presents a flexible configuration of QuickSwap that allows use of larger id columns, pressure pulse injections, and variable column flow rates without having to change the restrictor or QuickSwap pressure.

In this Application Note, the Agilent 6140 quadrupole MS, equipped with the multimode source, is combined with the Agilent 1200 Series Rapid Resolution LC system to develop a fast, generic LC/MS method for the analysis of 35 pharmaceutical compounds from 17 compound classes, reducing the method run time from 2.1 to 0.8 minutes.

In this Application Note, the Agilent 6140 quadrupole LC/MS and Agilent 1200 Series Rapid Resolution LC systems are combined to demonstrate the need for a fast-scanning, quadrupole MS detector, fast positive/negative switching, and uncompromised MS spectra quality in ultra-fast-scanning mode. The source of peak broadening of the MS peaks is explained and several application examples are given.

This document discusses the 1997 FDA ruling 21 CFR Part 11 and its impact on the pharmaceutical industry. It also describes the compliance features of the LC/MSD Trap Security Pack 1.0 software and the compliance services and training offered by Agilent Technologies.

This Application Note describes how a S9 human liver homogenate was used to simulate oxidative phase 1 metabolism of the beta-blocking drugs propranolol, metoprolol and pindolol. Afterwards, the samples were partially purified by precipitation/extraction with trifluoroacetic acid and hexane, followed by analysis with an Agilent 1100 Series LC/MSD trap XCT system. Fully automated LC/MS and LC/MSn analysis allowed for identification of the metabolites produced by the liver homogenate.

Introduction to MALDI and its applications in protein and peptide analysis. Including the principle of MALDI, the analysis methods of MALDI, the advantages and disadvantages of MALDI

This technical note describes the new features of the Agilent 1100 Series LC/MSD Trap software Version 4.2, including the Dissect function, the MASCAT program, and the automatic gain calibration function.

This document describes the polarity switching technology in mass spectrometric analysis and the ability of a mass spectrometer to switch polarities during a single chromatographic peak. This capability allows for the detection of various polarity molecules in unknown samples during a single run, saving time and improving data quality.

This document discusses the importance of target analysis of pharmaceutical compounds in biological matrices, and demonstrates the use of an ion trap LC/MS/MS system for fast and sensitive quantitation of the benzodiazepine drug midazolam in human serum.

This application note describes a method for impurity profiling using capillary electrophoresis (CE) and ion trap mass spectrometry (IT).

This document describes the method of rapid protein characterization using microflow LC/MS/MS techniques. The method involves digestion of a protein followed by microflow LC separation of the peptide fragments. Each peptide fragment is automatically selected for collision induced dissociation (CID) using mass spectrometric techniques, including automated data-dependent scan functions. Protein identification is achieved by correlating uninterpreted protein and peptide tandem mass spectral data with sequences in protein or nucleotide databases using computer algorithms. This method offers the potential for automated high-throughput data analysis and provides a high degree of specificity to database searches due to the unique fingerprint-type pattern displayed by the tandem mass spectrum representing every amino acid sequence.

This note describes the effect of coeluting internal standard concentration on the dynamic range of Ion Trap quantitative analyses and demonstrates the advantages of Ion Trap technology over triple-quadrupole instruments for quantitative applications in high-throughput environments.

This application note describes the use of an Ion Trap LC/MS/MS intelligent database search to identify phosphorylation sites in peptides resulting from proteolytic digestion of a phosphorylated protein

This application note describes a method for determining the metabolic stability profile of buspirone using an Agilent 1200 Series Rapid Resolution LC/TOF system on a short Rapid Resolution High Throughput (RRHT) column. Simultaneously, the main metabolites are identified in the same experiment by the automated use of a long RRHT column, which provides the necessary high resolution.