Agilent Manuals

accurately measure particle sizes of polymers, applications in coatings, paints, adhesives, drug delivery systems and medical diagnostics

This document describes a method for analyzing trace hydrocarbon impurities in benzene using the Agilent 7820A gas chromatograph. The method is simple to use and can be used to analyze benzene and other aromatic solvents for impurities.

This document introduces a method for routine detection and quantification of biomarkers in petroleum using the Agilent 7890A/7000A Series Triple Quadrupole GC/MS with backflushing using a Pressure Controlled Tee configuration. In a single run, diverse biomarkers from several transitions can be detected, confirmed, and quantified at levels as low as 2 ppm, with RSDs well below 5%. This method is suitable for “fingerprinting” of petroleum samples and the deconvolution of oil mixtures in complex, multisource petroleum systems.

This document introduces an economical solution to test permanent gases and light hydrocarbons using the Agilent 7820A GC 3-valve system. The Agilent 7820A Gas Chromatograph equipped with three valves, a flame ionization detector (FID), and a thermal conductivity detector (TCD), is configured for analysis of permanent gases and light hydrocarbons. The TCD channel with packed columns is used to measure H2,CO2,O2, N2,CH4 and CO. A capillary column ( Al2O3 PLOT: 50 m × 0.53 mm) is used to measure all hydrocarbons (C1~C6) including CH4.

This application note successfully demonstrates the selective retention and resolution of low molecular weight sulfur containing species on an Agilent J&W GS-OxyPLOT column. The selective retention for sulfur species enables enough of a retention shift away from hydrocarbon matrices such as base gasoline and n-butane to demonstrate the feasibility of effective quantification of sulfur species in process streams. Using an Agilent J&W GS-OxyPLOT column it is possible to shift the retention of sulfur containing analytes away from a hydrocarbon matrix to achieve effective quantification in the low ppm range.

This application note describes the use of an Agilent 6460 triple quadrupole LC/MS equipped with Agilent Jet Stream technology and direct aqueous injection for the analysis of a suite of acidic herbicides in several water matrices, thereby negating the need for solid phase extraction. Standard deviations in the range of 2.5% to 6.6% for analyte precision and recoveries of between 91.5% and 105.1% were observed over 11 batches of samples. Limits of detection were less than 10 ng/L (10 ppt) for all the compounds in the suite thus meeting current industry standards.

This application note describes a method for the analysis of impurities in fine chemical octyl-dimethyl-4-aminobenzoate using the Agilent 1290 Infinity LC and ZORBAX RRHT and RRHD 1.8 µm columns

This document introduces the advantages of Agilent 7700x ICP-MS technology in drinking water analysis. This technology uses the 3rd generation Octopole Reaction System (ORS3) technology to effectively remove polyatomic interferences. He mode is simple and easy to use, and the 7700x ICP-MS can remove all polyatomic interferences in a single set of conditions without requiring any prior knowledge about the sample, and without the need for complex mathematical corrections. This makes setup easier, detection limits are lower, analysis is faster, and interference removal is improved, especially for the difficult plasma-based interferences affecting iron and selenium. Currently, Se detection limits of less than 20ppt are routinely achievable in He mode.

This Application Note demonstrates the use of rapid resolution liquid chromatography in conjunction with tandem mass spectrometry to study the plasma stability of phar- maceutical compounds belonging to four different drug classes. The Agilent 1200 Series Rapid Resolution LC system (RRLC) provided fast separation of compounds and internal standard in all samples. The Agilent MassHunter Optimizer software identi- fied compound-specific MS parameters such as the most abundant product ions, opti- mal fragmentor voltages and collision energies. Taken together, robust methods for a larger set of compounds could be developed within a short period of time.

A profile-directed biomarker discovery approach involving initial differential expression analysis followed by targeted identification of differentially expressed proteins was used in this study. Using Agilent platforms such as a Multiple Affinity Removal (MARS) column, a 1200 Series HPLC-Chip/MS and a 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS, excellent reproducibility in retention time and intensity measurement, and outstanding accuracy in mass measurement and sensitivity over a wide dynamic range were achieved. Differential analyses using MassHunter Mass Profiler and MassHunter Mass Profiler Professional (MPP) software enabled the comparison between spiked and non-spiked plasma samples. Subsequent targeted MS/MS analysis on a Q-TOF LC/MS followed by a database search using Spectrum Mill helped identify the spiked protein.

This article introduces a fast GC-FID method for the analysis of mineral oil in environmental samples using a low thermal mass system. The fast oven temperature programming achieved by the low thermal mass system dramatically reduced the analysis cycle time and increased the sensitivity. The regulated method requirements are met for the environmental analysis of the C10-C40 hydrocarbon fraction in soil and water extracts using splitless injection with an analysis time less than three minutes. Cool-down time is less than two minutes, resulting in an injection-injection cycle time of five minutes. Method performance criteria, including repeatability, linearity and solute discrimination, are presented.

This note describes the Pressure Controlled Tee (PCT) configuration for rapid and universal GC/MS backflushing applied to selected reaction monitoring experiments on the Agilent 7890 GC used with the Agilent 7000 Series Triple Quadrupole Mass Spectrometer. This means that rapid backflushing and quick column and inlet maintenance without venting the MS system can be incorporated without a loss in detection limits using a simple PCT configuration.

Peptide Mapping of a Monoclonal Antibody using a Microfluidic-based HPLC-Chip coupled to an Agilent Accurate-Mass Q-TOF LC/MS

This document describes the analysis of 19 pharmaceuticals in positive ion mode and 11 pharmaceuticals in negative ion mode using solid phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The analysis was performed at low picogram level on column without any derivatization. Good linearity was observed for analytes from 1 pg to 1 ng on column. Repeatability from six injections of analytes at 5 pg on column showed RSDs below 15%, for all target compounds except for fluoxetine at 23%.

This application note describes a fast HPLC method for the analysis of DNPH derivatized carbonyls found in air samples. The method employs a 1.8-μm StableBond C18 column and a UV detector, and can separate and identify a standard of the major 13 DNPH derivatized carbonyls. An air sample is analyzed in under 10 minutes and found to contain several carbonyls.