Agilent Manuals

This Application Note describes the development of a rapid resolution LC method for the separation of the Chinese herbal prescription Zuo Jin Pill into its six major con- stituents: magnoflorine, jatrorrhizine, coptisine, palmatine, berberine and evodiamine. The method uses an Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system coupled with an Agilent 1200 Series Diode Array Detector (DAD). The separation was carried out on an Agilent ZORBAX Eclipse Plus C18 column (4.6 × 150 mm, 1.8 µm). The linear gradient was eluted with the mobile phase of an acetoni- trile – acetate buffer. All calibration curves showed good linearity (r2 >0.9998) within the test range 1.2-1200 µg/ml. The results demonstrate that this analytical method is simple, sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs. It is useful for the comprehensive evaluation of the quality of this traditional Chinese Medicine (TCM).

This article introduces a method for the high-pressure and high-flow analysis of polyaromatic hydrocarbons using the Agilent 1290 Infinity LC and Eclipse PAH columns.

Extended ionization capability of thermal gradient focusing ESI in high-throughput in-vitro ADME assays

An Application Kit for the Screening of Samples for Analytes of Forensic and Toxicological Interest using LC/QQQ MS/MS with a Dynamic MRM Transition Database

The present study developed a miRNA microarray analysis method suitable for tissue pathology applications. This method uses the miRNA Complete Labeling and Hyb Kit to extract miRNAs from FFPE samples, and then miRNAs are analyzed using a miRNA microarray. The results showed that this method can effectively extract miRNAs from FFPE samples and accurately analyze miRNA expression. This method provides a feasible method for miRNA analysis using FFPE samples.

The document describes the application of the Agilent Bravo Automated Liquid Handling Platform in quantitative polymerase chain reaction (QPCR) assays. It has been shown that the Bravo platform can effectively automate the setup of various QPCR assays and has similar slopes and good linearity compared to manual setup. Dilution samples set up by both methods showed similar slopes and good linearity, and the target genes amplified by both methods also had similar Ct values. When examining cDNA from fetal and adult lung tissues, differential expression was observed in the B2M gene, while expression of the GUSB and TBP genes remained similar. The Bravo Automated Liquid Handling Platform is useful for scientists who want to speed up setup while maintaining reliability in QPCR studies.

This application note provides detailed information about the Agilent BioCel system configuration for DNA purification and sequencing applications. The system consists of the Agilent Vertical Pipetting Station high-precision high-speed pipetter, which with its versatile shelf configuration options is ideally suited for complex pipetting sequences while simultaneously achieving very high throughput. The system can process 200 prepared DNA plates in four hours, eliminates sample contamination and ensures the highest possible quality of sequencing preparations.

This Application Bulletin describes a protocol for the Promega P450-Glo kit using the Agilent Bravo Platform for the measurement of Cytochrome P450 activity. The protocol involves a two-step reaction, in which the P450 enzyme metabolizes a luminogenic substrate (step 1) into a luciferin product in the presence of an NADPH regenerating system. The addition of a luciferin detection reagent (step 2) stops the enzyme reaction and converts the luciferin product into a luminescent signal that is directly proportional to the enzyme activity.

The Agilent 1290 Infinity UHPLC is a new liquid chromatograph that can be used for environmental applications. The two important innovations of this instrument are 1.8-µm columns and fast gradient. These two innovations can improve the analysis efficiency and reliability.

Agilent 7820A GC System can be used to simply determine formic acid in acetic acid

The document describes the method of analyzing impurities in octyl-dimethyl-p-aminobenzoic acid using the Agilent 1290 Infinity LC system. The system is capable of operating with long, high resolution columns under conditions of rapid analysis, using low viscosity acetonitrile and higher viscosity methanol as solvent conditions.

ASTM Method D 5580-94 is a standard method for analyzing the aromatic content of gasoline. This method uses an Agilent 6890 Series gas chromatograph system, including the Agilent ChemStation and automatic liquid sampler. This method can analyze benzene, toluene, ethylbenzene, p/m-xylene, o-xylene (BTEX), C9+ aromatics, and total aromatics in gasoline.

This document introduces the use of AC Oxygenate Analyzer, including instrument configuration, analysis conditions and chromatograms.

This document describes the features and characteristics of the AC Benzene/Toluene in Gasoline Analyzer, which is used to determine the levels of benzene, methyl ethyl ketone (MEK), and toluene in gasoline.

This application note describes a method for using an Agilent 6890 gas chromatograph to determine trace (low ppm) levels of hydrocarbon impurities in high-purity ethylene and propylene.