The Toyobo QPK-212T SYBR® Green Realtime PCR Master Mix manual details components, primer design, detection, protocols for ABI PRISM® 7700 and Roche…
The SYBR® Green Realtime PCR Master Mix -Plus- (QPK-212T) is a 2× master mix for real‑time PCR that contains all necessary components except primers. It is designed for highly specific intercalation assays using SYBR Green I and includes Hot Start technology to reduce non‑specific amplifications.
The manual is organized into sections covering an introduction, component list, primer design guidelines, detection device compatibility, specimen types, detailed PCR protocols for ABI PRISM® 7700 and Roche LightCycler™, troubleshooting tips, and a list of related Toyobo products.
| Storage temperature | -20°C (protected from light) |
|---|---|
| Short‑term storage | 4°C for up to 2 months |
| Reaction volume (ABI PRISM protocol) | 50 µl |
| Primer concentration (ABI PRISM protocol) | 0.2‑0.6 µM |
| Primer concentration (LightCycler protocol) | 0.4‑0.8 µM |
| Annealing temperature range | 55‑65 °C |
| Number of reactions per kit (QPK‑212T) | 40 reactions (1 mL) |
| Primer length recommendation | 20‑30 nucleotides |
Set the annealing temperature between 55 °C and 65 °C, depending on the primer melting temperature.