This document is about the instructions for using the DNA Ligation Kit- Ligation high (Code No.:LGK-100). The kit has features such as excellent ligation efficiency, ability to use small amounts of liquid for reaction, and good stability. The document also provides examples of using the kit for insert ligation, LINKER ligation, and phage ligation. It also mentions the effect of salt concentration on ligation efficiency.
The Ligation high kit (Code No.: LGK-100) from Toyobo is a research‑grade DNA ligation reagent designed for high‑efficiency joining of DNA fragments. The manual explains storage conditions, preparation of reaction mixtures, and recommended incubation parameters.
Key sections cover the operation procedure, distinctive features such as high reaction efficiency and stability after repeated freeze‑thaw cycles, and several experimental examples (insert ligation, linker ligation, phage ligation) that illustrate optimal conditions and the impact of salt concentration. This manual is written in Chinese.
| Storage temperature | -20 °C or lower |
|---|---|
| Reaction temperature | 16 °C |
| Reaction time | 30 minutes |
| Volume ratio DNA:Ligation high | 1:1 or 1:0.5 |
| Stability after freeze‑thaw cycles | No loss of efficiency after 50 cycles |
The reaction should be carried out at 16 °C.
Incubate the mixture for 30 minutes.
Store the kit at –20 °C or lower.
Yes, mixing equal or half the volume of Ligation high with the DNA solution works.