Key facts
- Manuals
- 10
- Models
- 2
- Languages
- EN · JA · ZH
- Last added
- 2026-10-03
TOYOBO models by category
Featured TOYOBO manuals
Toyobo QPS-101T Probe qPCR Mix Instruction Manual
The THUNDERBIRD™ Probe qPCR Mix is a 2× master mix designed for real‑time PCR with TaqMan® probes. It contains all required reagents except the ROX reference dye, which is supplied separately, and includes hot‑start antibodies for improved specificity and efficiency. The kit is intended for research use only and is compatible with most block‑type and glass‑capillary real‑time cyclers.
The manual covers the product components, guidelines for primer and probe design, template DNA options, detailed reaction setup, recommended thermal cycling parameters for various instruments, related cDNA synthesis protocols, troubleshooting tips, and a list of related products and licensing information.
Covers: Introduction · Components · Primer/Probe design · Template DNA · Protocol · Related Protocol
Toyobo QPK-212T SYBR® Green Realtime PCR Master Mix Instruction Manual
The SYBR® Green Realtime PCR Master Mix -Plus- (QPK-212T) is a 2× master mix for real‑time PCR that contains all necessary components except primers. It is designed for highly specific intercalation assays using SYBR Green I and includes Hot Start technology to reduce non‑specific amplifications.
The manual is organized into sections covering an introduction, component list, primer design guidelines, detection device compatibility, specimen types, detailed PCR protocols for ABI PRISM® 7700 and Roche LightCycler™, troubleshooting tips, and a list of related Toyobo products.
Covers: Introduction · Components · Primer design · Detection · Specimens · Protocol
TOYOBO SEAP Reporter Assay Kit Manual
This manual describes the SEAP Reporter Assay Kit, including safety precautions for research‑only use, required reagents, and equipment such as a luminescence meter and 96‑well microplates. It outlines the step‑by‑step assay protocol, incubation conditions, and detection procedures.
Additional sections cover troubleshooting tips for low SEAP activity, common sources of error, and contact information for technical support and the manufacturer. This manual is written in Japanese.
Covers: Safety Precautions · Reagents and Materials · Assay Procedure · Instrument Setup · Troubleshooting · Manufacturer Contact Information
TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
The TOYOBO QPK-201 Realtime PCR Master Mix (SYBR Green) is a 2× master mix that includes SYBR® Green I, dNTPs, MgCl₂, Taq DNA polymerase, anti‑Taq monoclonal antibody, BSA and a passive reference. It is compatible with a range of real‑time PCR instruments such as LightCycler™ and ABI PRISM®7700.
The manual provides detailed steps for preparing reaction mixtures, recommended primer concentrations, thermal cycling conditions, and storage guidelines (‑20 °C long‑term, 4 °C for up to three weeks after thawing). A troubleshooting section addresses common issues like messy amplification curves, poor reproducibility, and unexpected signals in blank samples. This manual is written in Chinese.
Covers: Preface · Precautions · Product Contents · Required Reagents · Operation Steps · Frequently Asked Questions
Latest TOYOBO manuals
Toyobo QPS-101T Probe qPCR Mix Instruction Manual
User Manual 14 pages · 169 KB · ENThe Toyobo QPS-101T Probe qPCR Mix instruction manual provides components, protocol, cycling conditions, and troubleshooting for real‑time PCR using TaqMan…
Toyobo QPK-212T SYBR® Green Realtime PCR Master Mix Instruction Manual
User Manual 8 pages · 163 KB · ENThe Toyobo QPK-212T SYBR® Green Realtime PCR Master Mix manual details components, primer design, detection, protocols for ABI PRISM® 7700 and Roche…
TOYOBO SEAP Reporter Assay Kit Manual
User Manual 8 pages · 242 KB · JAThe TOYOBO SEAP Reporter Assay Kit manual provides safety notes, assay procedure, reagent details, and troubleshooting for the SEAP assay.
TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
User Manual 10 pages · 340 KB · ZHInstructions, precautions, and troubleshooting for TOYOBO QPK-201 Realtime PCR Master Mix (SYBR Green) used in quantitative PCR.
TOYOBO THUNDERBIRD Probe qPCR Mix Manual
TOYOBO MagExtractor-Plasmid User Manual
User Manual 21 pages · ZHThe TOYOBO MagExtractor-Plasmid manual provides step‑by‑step instructions, reagent details, and troubleshooting for plasmid DNA purification using the…
TOYOBO Nucleic Acid Purification Kit MagExtractor -Plant Genome- (Code No. NPK- 500 501) Manual
TOYOBO QPK-201/QPK-201T SYBR Green Realtime PCR Master Mix User Manual
User Manual 13 pages · 214 KB · ZHThe TOYOBO QPK-201/QPK-201T SYBR Green Realtime PCR Master Mix User Manual provides composition, usage instructions, and troubleshooting for the 2× master…
TOYOBO SYBR Green Realtime PCR Master Mix Plus QPK-212 User Manual
User Manual 13 pages · 263 KB · ZHInstruction manual for TOYOBO SYBR Green Realtime PCR Master Mix Plus (QPK-212) covering composition, required items, usage steps, troubleshooting, and…
Toyobo Ligation high (LGK-100) User Manual
User Manual 12 pages · 315 KB · ZHThe Toyobo Ligation high (LGK-100) user manual provides step‑by‑step instructions, features, and experimental examples for efficient DNA ligation.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in TOYOBO manuals
How should the THUNDERBIRD Probe qPCR Mix be stored?
Store the mix at –20 °C, protected from light for long‑term storage, or at 2‑8 °C for up to three months for short‑term use.
What can cause loss of linearity at high cDNA/DNA concentrations?
Inhibition by components in the cDNA/DNA solution, such as PCR inhibitors in the DNA sample, can cause loss of linearity; repurifying the DNA often resolves the issue.
What annealing temperature should be used in the PCR cycles?
Set the annealing temperature between 55 °C and 65 °C, depending on the primer melting temperature.
From: Toyobo QPK-212T SYBR® Green Realtime PCR Master Mix Instruction Manual
What safety precautions should be taken when using the kit?
The kit is for research use only; avoid clinical applications and follow general laboratory safety guidelines.
Why might the amplification curve be messy?
It can be caused by PCR instrument settings or by problems with the reagents.
From: TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
Why can quantitative values show poor reproducibility?
Both instrument configuration and reagent quality can affect reproducibility.
From: TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual