The Toyobo QPS-101T Probe qPCR Mix instruction manual provides components, protocol, cycling conditions, and troubleshooting for real‑time PCR using TaqMan…
The THUNDERBIRD™ Probe qPCR Mix is a 2× master mix designed for real‑time PCR with TaqMan® probes. It contains all required reagents except the ROX reference dye, which is supplied separately, and includes hot‑start antibodies for improved specificity and efficiency. The kit is intended for research use only and is compatible with most block‑type and glass‑capillary real‑time cyclers.
The manual covers the product components, guidelines for primer and probe design, template DNA options, detailed reaction setup, recommended thermal cycling parameters for various instruments, related cDNA synthesis protocols, troubleshooting tips, and a list of related products and licensing information.
| Storage temperature (long term) | -20°C, protected from light |
|---|---|
| Kit volume | 1 mL per kit (QPS-101T) |
| ROX reference dye | 50× ROX reference dye supplied separately |
| Hot‑start technology | Anti‑Taq DNA polymerase antibodies |
| Cycler compatibility | Works with most block‑type and glass‑capillary real‑time cyclers |
Store the mix at –20 °C, protected from light for long‑term storage, or at 2‑8 °C for up to three months for short‑term use.
Inhibition by components in the cDNA/DNA solution, such as PCR inhibitors in the DNA sample, can cause loss of linearity; repurifying the DNA often resolves the issue.