This document provides instructions for using the 08-06 Reporter Assay Kit -SEAP- (Code No.: SAK-101). The kit is designed for detecting SEAP (secreted alkaline phosphatase). It features resistance to endogenous alkaline phosphatase interference, high sensitivity, and quick assay completion.
This manual describes the SEAP Reporter Assay Kit, including safety precautions for research‑only use, required reagents, and equipment such as a luminescence meter and 96‑well microplates. It outlines the step‑by‑step assay protocol, incubation conditions, and detection procedures.
Additional sections cover troubleshooting tips for low SEAP activity, common sources of error, and contact information for technical support and the manufacturer. This manual is written in Japanese.
| Incubation time | 15–30 min |
|---|---|
| Microplate format | 96‑well plate |
| Detection measurement time | 3–10 seconds |
| Internal AP inhibitor volume | 20 nl |
The kit is for research use only; avoid clinical applications and follow general laboratory safety guidelines.