This document is an instruction manual for 08-01 Realtime PCR Master Mix(SYBR Green) Code No. QPK-201, 201T. It describes the features and characteristics of the product, including high applicability and the ability to perform high-speed thermal activation.
The TOYOBO QPK-201 Realtime PCR Master Mix (SYBR Green) is a 2× master mix that includes SYBR® Green I, dNTPs, MgCl₂, Taq DNA polymerase, anti‑Taq monoclonal antibody, BSA and a passive reference. It is compatible with a range of real‑time PCR instruments such as LightCycler™ and ABI PRISM®7700.
The manual provides detailed steps for preparing reaction mixtures, recommended primer concentrations, thermal cycling conditions, and storage guidelines (‑20 °C long‑term, 4 °C for up to three weeks after thawing). A troubleshooting section addresses common issues like messy amplification curves, poor reproducibility, and unexpected signals in blank samples. This manual is written in Chinese.
| Formulation | 2× master mix |
|---|---|
| Fluorescent Dye | SYBR® Green I |
| Additive | BSA |
| Reference | Passive reference |
| Enzyme Inhibitor | Anti‑Taq monoclonal antibody |
| Storage (long term) | -20 °C, light‑protected |
It can be caused by PCR instrument settings or by problems with the reagents.
Both instrument configuration and reagent quality can affect reproducibility.