This document provides instructions and precautions for using the 10-10 SYBR® Green Realtime PCR Master Mix -Plus- (Code No.QPK-212) product from TOYOBO CO., LTD. Life Science Department. It also emphasizes that the product is for research use only and should not be used for diagnostic or clinical purposes.
The SYBR® Green Realtime PCR Master Mix -Plus‑ (Code No.QPK‑212) is a 2× master mix for real‑time quantitative PCR, containing SYBR Green I, dNTPs, MgCl₂, Taq DNA polymerase, anti‑Taq monoclonal antibody and a passive ROX reference dye. It is designed for research use only and is compatible with a wide range of real‑time PCR instruments.
This manual provides the product composition, a list of additional reagents and equipment required, detailed preparation and cycling instructions for both ABI PRISM® and Roche LightCycler™ platforms, common troubleshooting tips, and references to related TOYOBO products. This manual is written in Chinese.
| Storage temperature | -20 °C (light‑protected) |
|---|---|
| Short‑term storage | 4 °C for up to 2 months |
| Reaction capacity | 200 reactions (50 µL) or 500 reactions (20 µL) |
Rinse the affected area with plenty of flowing water; if the eyes are affected, rinse thoroughly for several minutes and seek medical attention if irritation persists.
One 1 ml vial provides enough master mix for 200 reactions of 50 µL each, or 500 reactions of 20 µL each.