Teaching plan with safety guidelines, experimental procedures and data analysis for Thermo Scientific picoSpin 45/80 NMR spectrometers.
This document provides a series of laboratory teaching plans for the Thermo Scientific picoSpin 45 and picoSpin 80 nuclear magnetic resonance (NMR) spectrometers. Each chapter includes an introduction, safety precautions, pulse‑sequence details, step‑by‑step experimental procedures, data processing instructions, and results discussion.
The plans cover topics such as quantum‑mechanics background, Fischer esterification, acetyl anhydride hydrolysis in D₂O, ferrocene micro‑scale acylation, and simple distillation of a toluene‑cyclohexane mixture, with specific instructions for both 45 MHz and 80 MHz instruments. This manual is written in Chinese.
| Operating frequency | 45 MHz or 80 MHz |
|---|---|
| Pulse sequence | Standard 90° single‑pulse |
Use the TMS signal at 0 ppm or the CHCl₃ signal at 7.24 ppm as reference points.