The Thermo Scientific TransformAid Bacterial Transformation Kit User Manual provides protocols, storage instructions, and troubleshooting for rapid…
The TransformAid Bacterial Transformation Kit enables rapid preparation of chemically competent E. coli cells from overnight cultures or colonies. The kit includes C‑medium, two T‑solutions, and reagents for up to 20 or 40 transformations, with detailed component listings and a certificate of analysis confirming high transformation yields.
The manual outlines step‑by‑step transformation protocols from both overnight cultures and colonies, storage conditions for reagents, important handling notes, ligation procedures, recipe formulations for antibiotics and media, and a troubleshooting guide to address common issues such as low efficiency or background colonies.
| Transformation efficiency | ≥10⁶ transformants per µg plasmid DNA |
|---|---|
| Number of transformations per kit | 20 or 40 transformations |
| T‑solution storage temperature | -20 °C |
| C‑medium storage | 4 °C up to 2 months or -20 °C longer |
| Hands‑on time for preparation | 20 min |
Both T‑solution (A) and T‑solution (B) should be stored at –20 °C until the expiry date.
The kit provides either 20 or 40 transformations, depending on the kit version.
Typical yields are more than 10⁶ to 10⁷ transformants per µg of plasmid DNA.
Freezing at –70 °C is not recommended; cells should be stored at –20 °C or used fresh.