The Thermo Scientific EDC manual provides storage guidelines, coupling protocols, and two‑step reaction procedures for product numbers 22980, 22981, and 77149.
This manual describes EDC (1‑Ethyl‑3‑[3‑Dimethylaminopropyl] Carbodiimide Hydrochloride), a zero‑length cross‑linker used to join carboxyl and amine groups. It includes product descriptions, molecular weight, CAS number, and recommended storage conditions for each product variant.
The document outlines single‑step coupling procedures, a two‑step EDC/NHS (or thiol‑NHS) coupling workflow, required reagents and buffers, quenching steps, purification using Zeba desalting columns, and storage of the final conjugates. Technical tips, related Thermo Scientific products, and warranty information are also provided.
| Molecular weight | 191.7 |
|---|---|
| CAS number | 25952-53-8 |
| Product 22980 amount | 5 g |
| Product 22981 amount | 25 g |
| Product 77149 amount | 10 mg |
Product 22980 should be stored dry at –20 °C after receipt.
Incubate the reaction at room temperature for 2 hours.
2‑Mercaptoethanol (2‑硫基乙醇) can be added to quench excess EDC.