The TaqDNA Polymerase SL2601 product leaflet provides specifications, storage conditions, and recommended PCR reaction setup.
TaqDNA Polymerase SL2601 is a recombinant enzyme derived from Thermus aquaticus YTI, offering 5'→3' polymerase and 5'→3' exonuclease activities. It is supplied as 1000 U of enzyme (5 U/µL) in a 200 µL volume and is suitable for routine PCR, cloning of short fragments, and 3'‑end labeling.
The enzyme should be stored at –20 °C in the provided buffer (20 mM Tris‑HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % NP‑40, 0.5 % Tween‑20, 50 % glycerol) for up to two years. Recommended reaction conditions include 50 mM KCl, 10 mM Tris‑HCl pH 8.6, 0.1 % Triton X‑100, and 1.5 mM MgCl₂, with 0.5–1 µL enzyme per 50 µL reaction. This manual is written in Chinese.
| Unit activity | 1000 U |
|---|---|
| Molecular weight | ≈94 kDa |
| Storage buffer composition | 20 mM Tris‑HCl (pH 8.0, 25 °C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % NP‑40, 0.5 % Tween‑20, 50 % glycerol |
| Reaction buffer composition | 50 mM KCl, 10 mM Tris‑HCl (pH 8.6, 25 °C), 0.1 % Triton X‑100, 1.5 mM MgCl₂ |
Use 0.5–1 µL of the enzyme (5 U/µL) per 50 µL reaction for optimal performance.
Store the enzyme at –20 °C; it remains stable for up to two years.
One unit is the amount of enzyme that catalyzes incorporation of 10 nmol dNTP into an insoluble precipitate within 30 minutes at 74 °C.