One Step SYBR Ex Taq qRT-PCR Kit (Perfect Real Time) is designed for Real-Time One Step RT-PCR using SYBR Green Detection. The RT-PCR is performed in a single tube for simple operation and minimal risk of contamination. In addition, amplified products are monitored in real time and do not require electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA (i.e. RNA virus).
The Takara One Step SYBR Ex Taq qRT-PCR Kit (Perfect Real Time), catalog number RR067A, is designed for Real-Time One Step RT-PCR using SYBR Green detection. The kit allows for the synthesis of cDNA from RNA and subsequent PCR amplification in a single tube, minimizing contamination risk and eliminating the need for post-PCR electrophoresis. It is optimized for detecting small amounts of RNA, such as RNA viruses.
| Reaction System Volume | 50 μl |
|---|---|
| Recommended RNA Template | 10 pg - 100 ng |
| Primer Final Concentration | 0.2 μM |
| Enzyme Type | TaKaRa Ex Taq HS |
No, the specific primer for reverse transcription should be used; Random Primer and Oligo dT Primer cannot be used.
No, amplified products are monitored in real time and do not require electrophoresis after PCR.