This document provides instructions for using a rat 8-hydroxydeoxyguanosine (8-OHdG) quantitative detection kit. The kit is designed for quantifying the content of 8-hydroxydeoxyguanosine in rat serum, plasma, and other samples.
This manual provides step‑by‑step instructions for quantifying 8‑OHdG in serum, plasma, or other liquid samples using the ELISA kit. It describes the required reagents, preparation of standards, sample incubation, washing, color development, and measurement at 450 nm.
The document also lists required equipment (37 °C incubator, precision pipettes, disposable tips, distilled water) and important precautions such as avoiding cross‑contamination, proper storage of sealed plates, and handling of light‑sensitive reagents. This manual is written in Japanese.
| Standard concentrations | 80, 40, 20, 10, 2.3 pg/mL |
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Incubations are carried out at 37 °C, typically in a water bath or incubator for 30 minutes.
You need a 37 °C incubator, precision pipettes with disposable tips, distilled water, and sealed plates.
Add substrate A (30 µL) then substrate B (50 µL) to each well, mix for 30 seconds, and incubate at 37 °C for 15 minutes before stopping the reaction.