The Rat IL-10 ELISA kit is used for the in vitro quantitative detection of natural and recombinant IL-10 in rat serum, plasma or cell culture supernatant. The kit uses a double antibody sandwich ELISA method, which is simple to use and has good reproducibility.
This manual describes the Excellbio Rat IL-10 ELISA Kit (Cat No. ER004), which is intended for quantitative detection of IL-10 in rat serum, plasma, or cell‑culture supernatants. The kit uses a double‑antibody sandwich ELISA format and provides detailed steps from sample preparation to result calculation.
The document includes a list of kit components, required equipment, sample collection guidelines, reagent preparation, the complete assay procedure, and information on assay performance such as sensitivity, specificity, and repeatability. Proper storage and handling instructions are also provided to ensure reliable results. This manual is written in Chinese.
| Detection range (standard curve) | 0 – 2000 pg/ml |
|---|---|
| Sensitivity (LOD) | 15 pg/ml |
| Specificity | No cross‑reactivity with IL‑1,2,3,4,5,6,8,12, IFN, TNF, VEGF, TGF and ICAM |
| Repeatability (intra‑ and inter‑assay CV) | < 10 % |
| Storage temperature | 2 °C – 8 °C |
The kit can detect as low as 15 pg/ml of rat IL‑10.
It is suitable for rat serum, plasma, or cell‑culture supernatant.
Add 0.5 ml of diluent to the freeze‑dried standard, let stand 15 min, then dilute to the required concentrations.
Store the kit at 2 °C to 8 °C before use.