This document introduces the Cat No.ER004 Rat IL-10 ELISA Kit, which is suitable for quantitative detection of IL-10 concentration in rat serum, plasma or cell culture supernatant. The kit uses a sandwich ELISA method with high sensitivity and accuracy.
This manual describes the ELISA kit for quantitative detection of rat IL‑10 in serum, plasma or cell‑culture supernatants. It explains the sandwich HISA principle, lists all kit components, and provides guidance on sample collection and required equipment.
Detailed instructions cover reagent reconstitution, preparation of standard curves, incubation steps, washing procedures, color development, and result interpretation, along with storage and handling precautions to ensure reliable measurements. This manual is written in Chinese.
| Detection Range | 0 – 2000 pg/ml |
|---|---|
| Repeatability (Intra‑ and Inter‑plate CV) | < 10 % |
The kit can be used with rat serum, plasma, or cell‑culture supernatant.