This kit is used to quantify BF in rat serum, plasma, cell culture supernatant or other relevant biological fluids.
This manual describes the ELISA immunoassay kit for measuring rat B Factor (BF) in serum, plasma, cell culture supernatant, and other biological fluids. It explains the assay principle, which uses a coated microplate, biotin‑labeled antibody, HRP‑avidin, and TMB substrate to generate a colorimetric signal proportional to BF concentration.
The document provides detailed guidance on reagent composition, sample collection and storage, preparation of standards and dilutions, the complete assay procedure, plate washing methods, data calculation, and important precautions to ensure accurate results. This manual is written in Chinese.
| Detection Range | 31.2 ng/ml - 2000 ng/ml |
|---|---|
| Shelf Life | 6 months |
| Long‑term Storage Temperature | -20°C |
| Frequent‑use Storage Temperature | 2‑8°C |
The kit can detect B Factor concentrations from 31.2 ng/ml up to 2000 ng/ml.
Store the kit at -20 °C for long‑term storage, or at 2‑8 °C for frequent use.
The kit is intended for rat serum, plasma, cell culture supernatant, and other related biological fluids.
The kit remains valid for 6 months.