This kit is used to measure the levels of kisspeptin in rat serum, cell supernatant and related liquid samples
The kit is designed to determine rat kisspeptin concentrations in serum, cell culture supernatant and other liquid samples using a double‑antibody sandwich ELISA. Purified rat kisspeptin antibodies coat the microplate, and after adding sample, HRP‑labeled antibody forms an antibody‑antigen‑enzyme complex that is developed with TMB substrate.
Sample handling includes specific procedures for serum, plasma, urine, cell culture supernatant and tissue homogenates, followed by a step‑by‑step assay protocol: standard preparation, sample dilution, incubation, washing, addition of enzyme conjugate, color development, termination and absorbance reading at 450 nm. The manual also provides calculation methods, performance specifications, storage conditions and safety precautions. This manual is written in Chinese.
| Detection range | 0.5 ng/ml – 22 ng/ml |
|---|---|
| Linearity (R²) | ≥ 0.95 |
| Shelf life | 6 months |
The kit can detect kisspeptin concentrations from 0.5 ng/ml up to 22 ng/ml.
Serum, plasma, urine, cell culture supernatant, tissue homogenates and other liquid samples are supported.
Samples containing sodium azide (NaN₃) must not be used because it inhibits HRP activity.