Rat type III collagen quantitative EIA kit instruction manual
The manual describes the components of the Rat III Collagen Quantification ELISA kit, including a 96‑well plate, standard solutions, antibodies, and reagents. It provides guidance on sample collection, storage conditions, and preparation of the standard curve.
Detailed assay steps cover plate setup, serial dilutions, incubation at 37 °C, washing (4‑6 times), addition of TMB substrate, color development, stopping the reaction, and reading absorbance at 450 nm. The document also includes data analysis instructions, reference values, and safety precautions for reagent handling. This manual is written in Chinese.
| Plate type | 96‑well microplate |
|---|---|
| Standard concentrations | 100, 50, 25, 12.5, 6.25, 3.12, 1.56, 0 ng/ml |
| Reference value | <1.2 ng/ml |
The normal reference value is less than 1.2 ng/ml.