E0543r IgM ELISA kit is a quantitative assay for IgM in rat serum, plasma, cell culture supernatant or other relevant liquids. The kit uses a double antibody sandwich enzyme-linked immunosorbent assay. Purified antibodies are coated on microwell plates to form a solid-phase antibody. IgM, biotinylated anti-rat IgM antibody, HRP-labeled affinity reagent are added to the wells coated with single antibodies in sequence, and then thoroughly washed with TMB substrate. TMB is converted to blue by peroxidase catalysis and converted to the final yellow by acid. The color depth is positively correlated with the IgM content in the sample. The absorbance (OD value) is measured at 450nm using an enzyme spectrophotometer and the sample concentration is calculated.
The OS43r kit is a sandwich ELISA designed to quantify IgM in serum, plasma, cell‑culture supernatant or other liquid samples. The assay uses a capture antibody coated plate, a biotin‑labeled detection antibody, HRP conjugate and TMB substrate, with absorbance measured at 450 nm.
The document details reagent preparation, step‑by‑step incubation and washing procedures, calculation of concentrations from standard curves, and important precautions such as proper washing, avoiding hemolysis, and storage conditions. This manual is written in Chinese.
| Detection range | 3.6 ng/mL – 1600 ng/mL |
|---|---|
| Storage temperature | -20 °C (long‑term) or 2‑8 °C for frequent use |
The assay can detect IgM concentrations from 3.6 ng/mL up to 1600 ng/mL.
Reagents should be stored at –20 °C, or at 2‑8 °C if they are used frequently.
It is a sandwich ELISA that uses a capture antibody coated plate, a biotin‑labeled detection antibody, HRP conjugate and TMB substrate to measure IgM levels.