Rat p-substance (SP) enzyme-linked immunosorbent assay kit user manual, can be used for quantitative measurement of SP content in rat serum, plasma, cell culture supernatant or other relevant biological fluids
This manual describes the SP ELISA immunoassay kit for quantitative measurement of rat SP in serum, plasma, cell culture supernatants and other biological fluids. It details the kit components, storage conditions, and the step‑by‑step assay procedure, from sample preparation to color development and absorbance reading at 450 nm.
The document also provides guidance on sample collection, dilution principles, preparation of standards, washing steps, and calculation of concentrations using the standard curve. Important precautions such as avoiding cross‑reaction, preventing foaming, and proper plate handling are highlighted to ensure reliable results. This manual is written in Chinese.
| Detection range | 1.56 pg/ml – 100 pg/ml |
|---|---|
| Limit of detection | 0.39 pg/ml |
| Specificity | No cross‑reaction with related proteins |
| Shelf life | 6 months |
| Assay type | ELISA (enzyme‑linked immunosorbent assay) |
| Sample types | Serum, plasma, cell culture supernatant, other biological fluids |
The kit can detect SP concentrations from 1.56 pg/ml up to 100 pg/ml.
Reagents should be stored at –20 °C for long‑term storage and at 2‑8 °C when used frequently.