Procedure guide for sampling Olympus flexible bronchoscopes with 3M Clean-Trace ATP tests to verify manual cleaning.
This guide describes the recommended procedure for obtaining acceptable ATP samples from Olympus® flexible bronchoscopes after manual cleaning. It outlines the materials, test points, and steps needed to assess residual clinical soil using 3M™ Clean-Trace™ ATP Surface and Water tests.
The document details how to prepare the bronchoscope, collect samples from the exterior surface and suction/biopsy channel, and interpret relative light unit (RLU) results. It also lists required equipment such as the Clean-Trace Luminometer, ATP test kits, syringes, and PPE, and provides reference FDA safety communications.
| RLU acceptance threshold | 200 |
|---|---|
| Sample swab length | 10 cm |
| Sterile water volume drawn | 40 cc |
An RLU value below 200 is considered acceptable and allows the process to continue.
The distal end should not go below the 40 mL mark in the collection tube to avoid contamination.