Thermo Scientific GeneJET Endo-free Plasmid Maxiprep Kit is a kit for extracting high-copy number plasmid DNA
The GeneJET Endo-free Plasmid Maxiprep Kit is designed for the large-scale isolation of high-quality endotoxin-free plasmid DNA from recombinant E. coli cultures. It utilizes silica-based membrane technology in the form of convenient spin columns to reduce endotoxin levels to less than 0.1 EU/µg of plasmid DNA.
| Maximum plasmid DNA recovery | up to 1000 µg |
|---|---|
| Endotoxin reduction level | <0.1 EU/µg |
| Recommended culture volume | 350 mL |
| Recommended cell density | OD 600 = 2-3 |
| Centrifuge speed for low speed protocol | 4,000-5,000 × g |
| Centrifuge speed for high speed protocol | ~48,000 × g |
Each preparation can recover up to 1000 µg of high copy plasmid DNA.
The kit is designed to reduce endotoxin levels to less than 0.1 EU/µg of plasmid DNA.
High quality plasmid DNA can be obtained from strains including DH10B, DH5α, XL1-Blue, JM109, JM107, and TOP10.
The recommended optical density for plasmid DNA isolation is approximately 2-3 (OD 600 = 2-3).