SuperSignal West Pico Chemiluminescent Substrate is a highly sensitive enhancer substrate used for detecting horseradish peroxidase (HRP) on immunoblot membranes.
This document provides instructions for using the SuperSignal West Pico chemiluminescent substrate, which is designed for high-sensitivity detection of horseradish peroxidase (HRP) on immunoblots. The kit includes luminol/enhancer and stable peroxide solutions suitable for detecting picogram quantities of antigen. This manual is written in Chinese.
| Detection Sensitivity | picogram quantities of antigen |
|---|---|
| Kit Volume (Large) | 250ml Luminol/Enhancer, 250ml Peroxide |
| Kit Volume (Small) | 25ml Luminol/Enhancer, 25ml Peroxide |
| Storage Condition | Room temperature |
| Substrate Stability | 8 hours at room temperature |
Avoid using sodium azide as a buffer preservative because it is an HRP inhibitor.
The substrate working solution is stable for 8 hours at room temperature.