This document details a workflow for identifying metabolites in complex biological matrices, specifically human plasma, using Gas Chromatography/Mass Spectrometry (GC/MS). The authors demonstrate the use of the Agilent Fiehn GC/MS Metabolomics RTL (Retention Time Locking) library to improve the reliability of metabolite annotation. The process involves protein precipitation, chemical derivatization, and analysis using specific GC/MS conditions. This manual is written in Japanese.
| Mass Range | 30 ~ 1.500 Da |
|---|---|
| Column Length | 29m |
| Column ID | 0.25 mm |
| Carrier Gas | Helium |
| Ion Source Temperature | 239°C |
| Library Size | Approx. 700 compounds |
It is developed to make metabolite identification easier, particularly by using retention time locking to improve identification reliability.
d27-myristic acid was used as the internal standard for locking the absolute retention time.