This document describes a method to reduce solvent consumption by decreasing the inner diameter (ID), length, and particle size of LC columns, resulting in a maximum reduction of 86% in solvent consumption and a maximum reduction of 67% in analysis time. The experiment used ZORBAX Eclipse Plus C18 columns for isocratic separation of a diet carbonated beverage and gradient analysis of a hickory smoked seasoning. By replacing the column with reduced ID, length, and particle size, it is demonstrated that solvent consumption can be reduced and analysis time can be shortened.
This document describes a method to reduce solvent consumption and analysis time in High Performance Liquid Chromatography (HPLC) by reducing the internal diameter (ID), length, and particle size of the column. It specifically focuses on the ZORBAX Eclipse Plus C18 column series. This manual is written in Japanese.
| Maximum solvent consumption reduction | 86% |
|---|---|
| Maximum analysis time reduction | 67% |
| Particle size for RR method | 3.5 nm |
| Particle size for RRHT method | 1.8 um |
| Solvent reduction with 3.0 mm ID column | 50% or more |
Solvent consumption can be reduced by up to 86%.
Analysis time can be shortened by up to 67%.
The ZORBAX Eclipse Plus C18 column is used.