Update: 29 September, 2023
This document describes a method to reduce solvent consumption by decreasing the inner diameter (ID), length, and particle size of LC columns, resulting in a maximum reduction of 86% in solvent consumption and a maximum reduction of 67% in analysis time. The experiment used ZORBAX Eclipse Plus C18 columns for isocratic separation of a diet carbonated beverage and gradient analysis of a hickory smoked seasoning. By replacing the column with reduced ID, length, and particle size, it is demonstrated that solvent consumption can be reduced and analysis time can be shortened.
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MD5 Checksum: 7AA0D1C657EAAF2E774E59B27835CFBB
Publication date: 06 July, 2012
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PDF Link: Agilent Reduction of solvent consumption by the size of the column Manual PDF