This document describes the application of Agilent 1200 series Evaporative Light Scattering Detector (ELSD) for the analysis of herbal preparations (Chinese herbal raw materials). HPLC separation methods were optimized for five herbal medicines and their formulations, and sample pre-treatment methods were also explained.
This application note details the method development for analyzing Radix Bupleuri (Shakuyaku), a traditional Chinese medicine, using an Agilent 1200 Series system. The study compares conventional HPLC analysis with Rapid Resolution LC (RRLC) to optimize analysis time and cost while maintaining separation efficiency. This manual is written in Japanese.
This document explains the method development for analyzing Radix Bupleuri using both traditional HPLC and Rapid Resolution LC (RRLC) analysis.
The optimized parameters for the ELSD were an evaporation temperature of 40 °C, a pressure of 55 psi, a gain of 7, and a filter of 3s.
According to reference 23, the analysis time for separating multiple saponins using traditional HPLC methods is approximately 50 minutes.