This Technical Overview demonstrates the performance of the Agilent 1100 Series nanoflow LC system for MS in protein identification. It discusses the features of the nanoflow pump and micro well-plate autosampler.
This document provides a technical overview of the Agilent 1100 Series Nanoflow LC system designed for mass spectrometry (MS) applications in proteomics. It focuses on the performance of the nanoflow pump and micro well-plate autosampler for identifying unknown sample proteins through LC/MS/MS analysis.
The system includes specific configurations for direct injection and enrichment injection modes. Key components discussed include the nanoflow pump with Electronic Flow Control (EFC), the micro well-plate autosampler, the thermostatted column compartment, and the Agilent Orthogonal Nanospray Source. The document details the advantages of the orthogonal nanospray source, such as easy assembly and reduced background noise, as well as the performance metrics of the nanoflow pump.
| Delay volume (nanoflow sensor end) | 300 nL |
|---|---|
| Delay volume (flow sensor) | 160 nL |
| Delay volume (capillary) | 140 nL |
| Primary flow path delay volume | 398 pL |
| Gradient delay time | about 2 minutes |
| Counter electrode voltage | 500 V below MS inlet |
| MS scan acquisition time | 1.25 seconds |
It is especially developed for the use in proteomics research to analyze complex digested protein samples.
The system supports direct injection mode and enrichment injection mode.