Agilent Rapid Resolution HT HPLC Columns Application Examples

About this manual

This document presents application examples for the new family of Rapid Resolution HT HPLC and LC/MS columns. These columns are packed with 1.8 µm particles, designed to provide very fast high-resolution separations on short lengths (15-50 mm). They are available in ZORBAX C18 bonded phases compatible with mobile phases from pH 1-9.

What's inside

• Technical Overview
• Fast LC and LC/MS Analysis of Antibiotics
• Increasing Sample Throughput Using Sub-Two Micron Particles
• The Influence of Sub Two-Micron Particles on HPLC Performance

Frequently asked questions

What are the dimensions and particle size of the Rapid Resolution HT columns described in the examples?

The columns are very short, measuring 15-50 mm in length, and are packed with 1.8 pm particles.

What is the maximum reduction in analysis time achievable with these columns compared to traditional ones?

The Rapid Resolution HT columns show up to an 80% reduction in analysis time compared to traditional sized columns.

How does the efficiency of a Rapid Resolution HT column compare to a column with 3.5 pm particles of the same dimensions?

The Rapid Resolution HT columns are designed to give twice the efficiency of a 3.5 pm column with the same column dimensions.