This document introduces the features and characteristics of Agilent OligoPore Columns (THF), including column design, use methods, performance advantages, etc.
This document provides a technical overview of the calibration process for Agilent OligoPore columns used in gel permeation chromatography. These columns feature an innovative medium with significantly increased pore volumes compared to conventional low pore size columns, resulting in higher resolution specifically for the oligomeric region and analysis of low molecular weight oligomers in organic solvents.
The document details a typical calibration procedure using polystyrene narrow standards in tetrahydrofuran (THF). It includes specific experimental conditions such as column dimensions, flow rate, injection volume, and detection method. The text presents data on resolved lower molecular weight polystyrenes and illustrates the resulting linear calibration curve up to the exclusion limit.
| Column Dimensions | 2x OligoPore, 300 x 7.5 mm |
|---|---|
| Part Number | PL1113-6520 |
| Sample Concentration | 0.1% (w/v) |
| Eluent | THF |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 100 pL |
| Detection Method | RI |
They are designed specifically for the analysis of low molecular weight oligomers in organic solvents.
Polystyrene narrow standards in tetrahydrofuran (THF) are one of the most common combinations.
The lower molecular weight polystyrenes 1270 and 580 have been resolved into individual oligomers.