Agilent OligoPore Columns DMF Calibration Technical Overview

This document introduces the simple calibration method of Agilent OligoPore Columns (DMF), using the integrated Agilent PL-GPC 220 GPC/SEC system, using Agilent polyethylene glycol (PEG) narrow standards at 80 °C

Brand: Agilent
Document type: Product Leaflet
Language: English
Pages: 2
File format: PDF
File size: 527 KB
Published: 05 July, 2012
Updated: 30 September, 2023
MD5 checksum: 8A1CBB1FC9184256F48A46D77CCE4C15
PDF: Agilent OligoPore Columns DMF Calibration Technical Overview PDF

About this manual

This document provides a technical overview of the calibration procedure for Agilent OligoPore columns when used with dimethyl formamide (DMF) containing 0.1% LiBr. The process utilizes Agilent polyethylene glycol (PEG) narrow standards at an elevated temperature of 80 °C within the integrated PL-GPC 220 GPC/SEC system to ensure high resolution in the oligomeric region.

What's inside

Introduction
OligoPore columns features
Solvent compatibility
Calibration conditions
Chromatogram results

Frequently asked questions

What temperature is required for calibrating OligoPore columns in DMF?

The calibration procedure uses a temperature of 80 °C.

What is the specified flow rate for the OligoPore column conditions?

The recommended flow rate is 1.0 mL/min.

How much sample should be injected during calibration?

An injection volume of 100 pL is required for the samples.

Download PDF (527 KB)