This document is about Agilent PLgel MIXED Columns technical overview
This document provides a technical overview of Agilent PLgel MIXED gel columns, which are designed for analyzing polydisperse materials by combining individual pore size materials within a single column. This approach eliminates the spurious peak shapes and precision loss associated with traditional series combinations of mismatched calibration curves.
The text also discusses extra-column dispersion in chromatographic systems, noting that while detector cell volume is at the manufacturer's discretion, users can optimize efficiency by controlling extra volume in connectors and injection/detection systems. A specific experimental setup using PLgel MIXED-D and MIXED-E columns with THF eluent is detailed.
These columns contain a mixture of individual pore size materials blended to cover a broad molecular weight range with a linear calibration, which eliminates column mismatch and avoids spurious peak shapes.
The PLgel 5 um MIXED-D column is 300 x 7.5mm, while the PLgel 3 um MIXED-E column is also 300 x 7.5 mm.
The system should use THF as the eluent at a flow rate of 1.0 mL/min.