Agilent Fast HPLC for Large Biomolecules Technical Overview

About this manual

This document provides a technical overview of Agilent chromatographic media designed for the analysis of large biomolecules. It details how specific pore sizes (1000A and 4000A) maximize selectivity and capacity across various molecule sizes, from di-peptides to large proteins.

The text presents experimental data demonstrating high-speed separations using PL-SAX, PL-SCX, and PLRP-S columns. Examples include the separation of ovalbumin and soyabean trypsin inhibitor, globular proteins like myoglobin, and rapid reversed-phase analysis of six protein test probes in under 60 seconds.

What's inside

• Introduction
• Ion-exchange separation using PL-SAX
• High speed separation on PL-SCX
• Reversed-phase protein analysis on PLRP-S
• High speed peptide analysis

Frequently asked questions

Which pore size is preferred for high resolution and very large biomolecules?

The 4000A material is preferred for high resolution, high speed applications, or the separation of very large biomolecules.

What is the flow rate used in Figure 6 for separating six protein test probes?

The flow rate used was 4.0 mL/min.