Bond Elut PBA is a unique silica SPE sorbent containing a phenylboronic acid functionality that can retain analytes via a reversible covalent bond. This very strong covalent retention mechanism enables high specificity and cleanliness. The boronate group has a strong affinity for cis-diol containing compounds such as catechols, nucleic acids, some proteins, carbohydrates and PEG compounds. Aminoalcohols, alpha-hydroxy amides, keto compounds, and others can also be retained.
This document provides a technical overview of the Bond Elut PBA silica SPE sorbent, which utilizes a unique reversible covalent bond mechanism to retain analytes containing cis-diols, such as catechols, nucleic acids, and carbohydrates. The text details the three-step process of boronate binding involving equilibration, retention, and elution, highlighting the high specificity of this covalent interaction compared to standard non-polar or ionic mechanisms.
The guide covers the types of molecules expected to bind to phenylboronic acid, including polyhydroxy compounds, aromatic o-hydroxy acids, and aminoalcohols. It further explains the extraction procedure, including cartridge conditioning with alkaline solutions, the selection of appropriate buffers to manage secondary interactions like charge-transfer complexes, and methods for removing interferences and eluting the compound of interest.
| pKa of immobilized phenylboronic acid | approximately 9.2 |
|---|---|
| Product Part Number | 12102019 |
It employs a reversible covalent interaction between the PBA and sample molecule(s).
Compounds containing cis-diols, such as catechols, nucleic acids, some proteins, carbohydrates, and PEG compounds.
Elution is achieved by acidification of the boronate complex, which releases the diol-containing compound.