This Technical Overview demonstrates the power of Agilent BioPack software to meet the needs of researchers in the field of biomolecular structure studies. The software provides a sophisticated turn-key approach that allows operators to select from over 350 sophisticated NMR pulse sequences, specify experiment time, and then generate high-quality and reproducible results.
This document provides a technical overview of the Agilent BioPack software package designed to facilitate easy, robust, and accurate Nuclear Magnetic Resonance (NMR) analysis of biomolecules. It highlights how the software simplifies complex protein structure studies by automating experiment setup and calibration.
The text details the capabilities of BioPack, which includes a library of over 350 sophisticated NMR pulse sequences for studying proteins, RNA, and DNA complexes. Key features include automatic calibration of pulse powers and water suppression, support for triple-resonance experiments on labeled samples, and automated projection reconstruction for faster data acquisition.
Triple resonance experiments are required for the sequential assignment of larger proteins that are greater than 150 amino acids.
BioPack provides a full suite of over 350 sophisticated pulse sequences to study proteins, RNA, or DNA complexes.
The Auto2D selections run approximately 37 2D experiments using triple-resonance pulse sequences to assess suitability for 3D.