Eclipse XDB-C8 and -C18 are microparticulate packings that use the technologies of eXtra-Dense Bonding (XDB) of organo-silane ligands and double endcapping to protect the ultra-pure (Type B) silica support from dissolution in mobile phases of intermediate pH. Eclipse XDB-C8 and -C18 are especially useful for the separation of acidic, basic, and other highly polar compounds by reversed-phase liquid chromatography. Eclipse XDB-C8 and -C18 are made by first chemically bonding a dense monolayer of dimethyloctyl (or octadecyl) silane stationary phase to a specially prepared, ultra-high purity ( 99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special ZORBAX silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded phase packing is then double endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse XDB-C8 and -C18 can be used in the pH range of 2–9, but is best suited for long-term operation at pH 3–8. The uniform, spherical Eclipse XDB-C8 and -C18 particles are based on ZORBAX Rx-SIL silica support that has a surface area of 180 m2/g and a controlled pore size of 80Å. Columns are packed to a stable uniform bed density using a proprietary, high-pressure, slurry-packing technique to give maximum column efficiency and longevity.
This document provides a data sheet for Agilent Eclipse XDB-C8 and -C18 microparticulate packings. These columns utilize eXtra-Dense Bonding (XDB) technology on ultra-pure silica supports to protect against dissolution at intermediate pH levels, making them suitable for separating acidic, basic, and highly polar compounds via reversed-phase liquid chromatography.
| pH Range | 2-9 (best 3-8) |
|---|---|
| Silica Support Surface Area | 180 m²/g |
| Controlled Pore Size | 80A |
| Maximum Column Temperature | <40 °C |
Typical wash volumes are 12.5 mL for a 75 mm length, 25 mL for a 150 mm length, and 41 mL for a 250-mm length.
The shipping solvent is water/acetonitrile or water/methanol.
Yes, the use of a cartridge guard column packed with ZORBAX (part number 7995118-504) is recommended.