This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix with the Stratagene Mx3000P and Mx3005P QPCR Systems. For detailed instructions, refer to the full product manual.
The document serves as a quick reference guide for the Stratagene Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix. It outlines the optimized protocol specifically designed for use with the Stratagene Mx3000P and Mx3005P QPCR Systems from Agilent Technologies.
| Reaction Volume | 20 µl |
|---|---|
| Primer Concentration Range | 150–500 nM |
| Total RNA Quantity Range | 0.1 pg – 100 ng |
| mRNA Quantity Range | 0.1 pg – 1 ng |
It provides an optimized protocol for use with the Stratagene Mx3000P and Mx3005P QPCR Systems.
The profile includes a 5-second denaturation step, though some assays may require up to 20 seconds.