This document provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QRT-PCR Master Mix with the Stratagene Mx3000P and Mx3005P QPCR Systems from Agilent Technologies. It also includes information about Agilent Technologies.
This document serves as a Quick Reference Guide for the Stratagene Brilliant III Ultra-Fast QRT-PCR Master Mix, designed specifically for use with the Stratagene Mx3000P and Mx3005P QPCR Systems from Agilent Technologies. It outlines an optimized protocol to assist users in performing real-time quantitative PCR (QRT-PCR) experiments efficiently.
| Reference Dye Dilution | 1:500 using nuclease-free PCR-grade water |
|---|---|
| Final Reaction Volume | 20 µl (including RNA) |
| Probe Concentration Range | 100–600 nM |
| Primer Concentration Range | 200–600 nM |
| Total RNA Quantity Range | 0.1 pg – 100 ng |
| mRNA Quantity Range | 0.1 pg – 1 ng |
Dilute the reference dye 1:500 using nuclease-free PCR-grade water.
The protocol suggests bringing the final reaction volume to 20 µl.