The AffinityScript One-Step RT-PCR Kit is a one-step RT-PCR kit that outperforms the competition with unparalleled yield, sensitivity, and specificity. It provides a complete system for fast, high-yield, reliable single-tube RT-PCR, ideal for gene profiling and other high-throughput end-point RT-PCR applications.
The Agilent AffinityScript One-Step RT-PCR Kit provides a complete system for fast, high-yield, reliable single-tube RT-PCR. It utilizes a master mix format coupled with robust performance and rapid cycling conditions, making it ideal for gene profiling and other high-throughput end-point RT-PCR applications.
| Target Length | up to 9.5 kb |
|---|---|
| Reverse Transcriptase Range | 42°C to 55°C |
| RNA Input | 1 ng |
| RNase Inhibitor | included |
The multiple temperature reverse transcriptase shows optimal activity over a broad temperature range, from 42°C to 55°C.
The kit routinely detects and amplifies targets using 1 ng of total RNA.
Yes, the kit allows for the amplification of multiple targets in one tube at the same time.