QuikChange Lightning Site-Directed Mutagenesis Kit is the next generation of mutagenesis technology from Stratagene, faster and easier to use than the original
The Agilent QuikChange Lightning Site-Directed Mutagenesis Kit represents the next generation of mutagenesis technology, designed to introduce point mutations, insertions, or deletions significantly faster than the original QuikChange kits. The kit utilizes specially engineered enzymes to shorten the signature three-step protocol, making it optimized for difficult-to-replicate sequences and large plasmids.
The document compares the performance of the new Lightning kit against the original QuikChange Mutagenesis Kit. It demonstrates that the Lightning kit reduces both thermal cycling and digestion times, resulting in a total process time that is up to 406% faster for certain template sizes compared to the original method.
The total time is 3 hours and 2 minutes for the Lightning kit, which is 343% faster than the original kit's 5 hours and 36 minutes.
The total time required for the QuikChange Lightning protocol is 3 hours and 2 minutes.
The total time required for the original QuikChange Mutagenesis Kit is 5 hours and 36 minutes.