Brilliant ROX! Brilliant II QPCR and QRT-PCR master mixes with High ROX have been specifically designed and optimized for use with all ABI real-time PCR systems
Agilent Technologies presents the Brilliant II QPCR and QRT-PCR Master Mixes with High ROX, specifically designed and optimized for use with all ABI real-time PCR systems. These ready-to-use reaction mixes contain the high ROX reference dye premixed at an optimal concentration, eliminating the need for additional pipetting steps while ensuring accurate reference dye correction.
The document details the performance benefits of the Brilliant II formulation, including improved sensitivity for detecting low copy numbers, earlier Ct detection, and consistent reproducibility across a wide dynamic range. It includes graphical data comparing the Brilliant II mix against a commercially available competitor on ABI PRISM 7900HT and StepOnePlus instruments, demonstrating superior efficiency and tighter replicates.
| Compatible Instruments | ABI real-time PCR systems |
|---|---|
| Document Type | Data Sheet |
| Publication Date | December 1, 2009 |
| Document Number | 5990-4950EN |
It is specifically designed and optimized for use with all ABI real-time PCR systems.
The formulation provides improved sensitivity, earlier Ct detection, consistent reproducibility, and higher efficiency and specificity.
The premixed optimal concentration eliminates an additional pipetting step and ensures the correct amount of ROX in the reaction.