This data sheet introduces the Agilent Protein Fractionation System and Proteomics Reagents for LC/MS analysis of biological samples, including antibody depletion, sample desalting, concentration, and fractionation.
The Agilent Protein Fractionation System and Proteomics Reagents are designed to facilitate the isolation and identification of proteins in biological samples such as serum, plasma, and cerebro-spinal fluid. The system includes the Multiple Affinity Removal System to eliminate high-abundance interfering proteins and the mRP-C18 High-Recovery Protein Column for simultaneous desalting, concentrating, and fractionating samples with high recovery rates.
| Recovery rate for mRP-C18 High-Recovery Protein Column | > 98 % |
|---|---|
| Recovery rate for membrane protein fractions | > 95 % |
| Protein removal efficiency for Multiple Affinity Removal System | approximately 94% |
| Number of high abundance proteins removed by Human 14 Columns | 14 |
| Number of runs for Multiple Affinity Removal Columns and Spin Cartridges | more than 200 runs |
| Number of proteins in Complex Proteomics Standard | over 1,500 proteins |
It is designed to chromatographically eliminate interfering high-abundance proteins from biological samples to improve subsequent LC/MS and electrophoretic analysis.
It is designed to simultaneously desalt, concentrate, and fractionate samples in one easy step with extremely high recovery.
Yes, these validated reagents are fully compatible with Agilent LC/MS methods and require no additional sample pretreatments.