Agilent Protein Fractionation System and Proteomics Reagents Data Sheet, this data sheet introduces the applications, introduction and principle of Agilent Protein Fractionation System and Proteomics Reagents
The Agilent Protein Fractionation System and Proteomics Reagents are designed to facilitate the isolation and identification of proteins in biological samples such as serum, plasma, and cerebro-spinal fluid. The system includes the Multiple Affinity Removal System to eliminate high-abundance interfering proteins and the mRP-C18 High-Recovery Protein Column for simultaneous desalting, concentrating, and fractionating samples with high recovery rates compatible with LC/MS analysis.
Additionally, the document describes validated proteomics reagents including a Complex Proteomics Standard, Proteomics Grade Trypsin, and FFPE Protein Extraction Solution. These reagents are intended for sample preparation in biomarker discovery and shotgun proteomics applications, offering compatibility with Agilent LC/MS methods without requiring additional sample pretreatments.
| Protein Recovery | > 98 % |
|---|---|
| Membrane Protein Recovery | > 95 % |
| High Abundance Protein Removal | approximately 94% |
| Number of Proteins Removed (Human) | 14 |
| Reagent Compatibility | Agilent LC/MS methods |
| Proteomics Standard Content | over 1,500 proteins |
It is designed to chromatographically eliminate interfering high-abundance proteins from biological samples to improve subsequent LC/MS and electrophoretic analysis.
It offers an easy, automated one-step solution that simultaneously desalts, concentrates, and fractionates samples with extremely high recovery.
Yes, they are fully compatible and require no additional sample pretreatments.
They are essential tools for sample preparation in biomarker discovery, protein characterization, and shotgun proteomics applications.