Stratagene Brilliant II QPCR and QRT-PCR Master Mixes with High ROX are specifically designed and optimized for use with all ABI real-time PCR systems. They provide improved performance with earlier Ct detection, consistent reproducibility across a wide dynamic range, increased sensitivity of detection to low copy numbers, higher efficiency, and specificity. The master mix kits come with high ROX reference dye premixed at optimal concentration for ABI instruments.
Agilent Technologies presents the Brilliant II QPCR and QRT-PCR Master Mixes with High ROX Concentration. These reagents are specifically designed and optimized for use with all ABI real-time PCR systems. The formulation provides performance benefits including earlier Ct detection, consistent reproducibility across a wide dynamic range, increased sensitivity to low copy numbers, and higher efficiency and specificity.
It is used for the real-time amplification of cDNA, genomic DNA, or plasmid DNA.
It detects the threshold cycle 1.8 to 2.8 cycles earlier than commercially available master mixes.
The mix achieves an amplification efficiency of 97% while consistently detecting these low copy number differences.