Agilent ZORBAX Rapid Resolution High Definition HILIC Plus Threaded Column is a high performance liquid chromatography column produced by Agilent company, which is mainly used for the high performance liquid chromatography separation of water soluble compounds. The column has a diameter of 2.1 mm, a length of 50 mm, and a particle size of 1.8 µm.
Agilent ZORBAX Rapid Resolution High Definition (RRHD) HILIC Plus threaded columns are nonbonded silica columns optimized for hydrophilic interaction chromatography (HILIC). They are specifically designed for use with ultra-high performance liquid chromatographs, such as the Agilent 1290 Infinity LC, and can operate up to a pressure of 1200 bar. The columns ship containing acetonitrile and water, ready for immediate HILIC separation.
| Maximum operating pressure | 1200 bar (17,000 psi) |
|---|---|
| Optimal lifetime pressure | up to 1000 bar |
| Maximum operating temperature | 40 °C |
| Operating pH range | pH 1 to 8 |
| Column type | Nonbonded silica columns |
The column must be equilibrated with 20 to 50 column volumes before use, typically starting with 30 to 40% water in acetonitrile.
These columns are best used for the retention and separation of small, polar analytes such as nucleosides, metformin, and acrylamide.
Acetonitrile:water (95%:5%) is recommended as the long-term storage solvent.